Characterization of spiny lobsters from Bangladesh waters using morphology, COI and 16S rRNA sequences

Md Sagir Ahmed,Tonmoy Saha,Anindita Barua,Sumaiya Ahmed,Durjoy Raha Antu,Sujan Kumar Datta

doi:10.1016/j.heliyon.2022.e08846

Md Sagir Ahmed, Tonmoy Saha + Show 4 more

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https://doi.org/10.1016/j.heliyon.2022.e08846

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Journal: Heliyon	Publication Date: Jan 28, 2022
Citations: 4	License type: cc-by

Affiliation: University of Dhaka, Jagannath University

Abstract

This study aims to taxonomically identify and characterise the phylogenetic relationships of spiny lobsters based on mitochondrial cytochrome c oxidase I (COI) and 16S rRNA genes from Bangladesh waters. A total of 19 barcode sequences (10 partial COI sequences and 9 partial 16S rRNA) were successfully generated from 12 collected spiny lobster samples representing four species belonging to the family Palinuridae. The average genetic distances within and between species were 0.834 ± 0.427 and 17.810 ± 0.830, respectively, in COI and 0.107 ± 0.255 and 8.401 ± 2.547, respectively, in 16S rRNA genes. The successful amplification rate of 16S rRNA was higher than that of the COI marker. In the maximum likelihood (ML) tree, the sequences of the same species were clustered together under a single clade for both COI and 16S rRNA, which supports the efficacy of both marker genes in differentiating lobster species.

Highlights

Lobsters are one of the most valuable and highly priced crustaceans in domestic and international markets
The present study aims to validate the morphologically identified lobster species based on mitochondrial cytochrome c oxidase I (COI) and the 16S rRNA gene
Morphometric key characteristics clearly identified spiny lobsters collected from the coastal region of Bangladesh (Figures 1, 2, 3, and 4), which is consistent with previous literature (Holthuis, 1991; Chan, 1998; Wahyudin et al, 2017)

Summary

Introduction

Lobsters are one of the most valuable and highly priced crustaceans in domestic and international markets. Four species of spiny lobsters (Panulirus homarus, P. ornatus, P. polyphagus and P. versicolor) and two species of slipper lobsters (Thenus orientalis and Scyllarus depressus) have been documented far from Bangladesh under the families Palinuridae and Scyllaridae, respectively (Ahmed et al, 2008; IUCN Bangladesh, 2015). Scyllarus depressus is a Western Atlantic species, it has been recorded from shallow rocky substrates and coral reefs of St. Martins Island, Naf River mouth (Teknaf) and the coast of Bangladesh (Ahmed et al, 2008; IUCN Bangladesh, 2015). Due to the high market price, spiny lobsters are more highly exploited than slipper lobsters in Bangladesh

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