Characterization of somatotropin- and prolactin-binding sites on bovine granulosa cells using homologous hormones.

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Specific binding of bovine somatotropin (BST) and bovine prolactin (BPRL) to cow granulosa cells from antrum-containing follicles of different diameter was studied. Scatchard analysis of the data revealed a single type of low affinity BST-binding sites on the granulosa cells with dissociation constants similar to those for the BPRL-binding sites. The number of BST-binding sites on the cells decreased with increasing follicle diameter from 3-5 to 6-10 mm. However, the binding capacity to BPRL decreased only in the case of cells from follicles 11-20 mm in diameter. The findings are discussed in relation to the "homologous binding" phenomenon.