Characterization of Silk/�-Tricalcium Phosphate Scaffolds for the Bone Tissue Engineering

Abstract

Event Abstract Back to Event Characterization of Silk/β-Tricalcium Phosphate Scaffolds for the Bone Tissue Engineering Gilson Khang1, Dae Hoon Lee1, Jae Hun Shin1, Jeong Eun Song1 and Chan Hum Park2 1 Chonbuk National University, BIN convergence technology, Korea 2 College of Medicine, Hallym University, Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Korea Silk is a potential material for biomedical applications because it has several attractive properties such as biocompatibility and biodegradability. However, it has a lack of mechanical properties for bone replacement graft material. To increase osteoconductivity and osteoinductivity, we modified the mechanical property and osteoconductivity of silk with β-Tricalcium Phosphate (β-TCP). β-TCP has degradable, osteoconductive and minimal cytotoxicity. We checked the physical and chemical characterizations of silk/β-TCP scaffolds using SEM, compressive strength, and FT-IR, etc. Also, osteogenic differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) cultured in scaffold was evaluated by using alkaline phosphatase (ALP), MTT, RT-PCR, and SEM. These results showed that silk/β-TCP (25:75) scaffold increased cell proliferation and differentiation of rBMSCs in vitro for 1 to 4 weeks. Also, histology result from implantation showed the same of them. From these results, it could be suggested that silk/β-TCP (25:75) scaffold is applied to one of useful biomaterials in terms of bone regeneration. Bio-industry Technology Development Program (112007-05-1-SB010), Technology Commercialization Support Program (814005-03-1-HD020), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. Keywords: Cell Differentiation, Regenerative Medicine, in vitro, biomaterial Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in constructing tissue substitutes Citation: Khang G, Lee D, Shin J, Song J and Park C (2016). Characterization of Silk/β-Tricalcium Phosphate Scaffolds for the Bone Tissue Engineering. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00362 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gilson Khang Dae Hoon Lee Jae Hun Shin Jeong Eun Song Chan Hum Park Google Gilson Khang Dae Hoon Lee Jae Hun Shin Jeong Eun Song Chan Hum Park Google Scholar Gilson Khang Dae Hoon Lee Jae Hun Shin Jeong Eun Song Chan Hum Park PubMed Gilson Khang Dae Hoon Lee Jae Hun Shin Jeong Eun Song Chan Hum Park Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.