Abstract
Abstract—Nuclear and polysomal brain RNA from the rabbit bind to Millipore filters and oligo(dT)‐cellulose suggesting the presence of poly(A) sequences. The residual polynucleotide produced after RNase digestion of 32P pulse‐labelled brain RNA is 95% adenylic acid and 200‐250 nucleotides in length. After longer isotope pulses the polysomal poly(A) sequence appears heterodisperse in size and shorter than the nuclear poly (A). Poly(A) sequences of brain RNA are located at the 3′‐OH termini as determined by the periodate‐[3H]NaBH4 labelling technique. Cordycepin interferes with the processing of brain mRNA as it inhibits in vivo poly(A) synthesis by about 80% and decreases the appearance of rapidly labelled RNA in polysomes by about 45%. A small poly(A) molecule 10‐30 nucleotides in length is present in rapidly labelled RNA. It appears to be less sensitive to cordycepin than the larger poly(A) and is not found in polysomal RNA.
Published Version
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