Abstract
To elucidate the chemical compositions of Prunus cerasifera Ldb leaves, the methanol extracts were firstly fractionated by ethyl acetate and n-butanol, respectively. The phenolic acid-rich fractions (ethyl acetate extracts) were further isolated by various chromatographic columns (CC) including MCI macroporous resin, normal-phase silica gel, Sephadex gel LH-20, octadecyl silane (ODS), and preparative HPLC to yield the phenolic compounds. The isolated compounds were analyzed by 1H-nuclear magnetic resonance (1H-NMR), 13C-NMR, and electrospray ionization mass spectral (ESI-MS) spectroscopy. Eleven phenolic acids were identified as p-coumaric acid (1), caffeic acid (2), ferulic acid (3), chlorogenic acid (4), 3-O-caffeoylquinic acid (5), 5-O-coumaroylquinic acid (6), 3-O-caffeoylquinic acid methyl ester (7), chlorogenic acid methyl ester (8), 3-O-caffeoyl-5-O-coumaroylquinic acid or 3-O-coumaroyl-5-O-caffeoylquinic acid (9), gallic acid (10), and protocatechuic acid (11). The current study pioneers to identify and report all the phenolic constituents from P. cerasifera Ldb leaves.
Highlights
Plums are a famous juicy and nutritious fruit cultivated throughout the world [1]
In China, P. cerasifera Ehrh. is native to the Tianshan area of the Xinjiang province and well liked by the community due to its higher nutrition values [1, 3]. e genus Prunus have extensively been studied for their phytochemical screening and reported to contain different polyphenols and their derivatives [4,5,6,7,8,9,10,11]
Electrospray ionization mass spectral (ESI-MS) data were tested by an AB-QTRAP 4500 mass spectrometer (ESI-MS/MS; Applied Biosystems, Foster City, CA)
Summary
Plums (genus Prunus of family Rosaceae) are a famous juicy and nutritious fruit cultivated throughout the world [1]. Various plum species are grown worldwide including Prunus domestica L., P. salicina Lindl., P. americana Marsh., P. cerasifera Ehrh., P. insititia L., and P. spinosa L. E genus Prunus have extensively been studied for their phytochemical screening and reported to contain different polyphenols and their derivatives [4,5,6,7,8,9,10,11]. P. cerasifera is an endangered fruit species grown wild in Southern slopes of the Huicheng area in the Xianjiang province of China [12]. Huge literature reported its biological significance, and it bears strong antioxidant potential along with biologically active ingredients the flavonoids, saponins, and various phenolic acids [15, 16]
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