Characterization of Nano-graphene oxide stabilized by Pluronic as a nanocarrier

Abstract

Event Abstract Back to Event Characterization of Nano-graphene oxide stabilized by Pluronic as a nanocarrier Dharshini Perumal1, Suhaili Shamsi1*, Faizah Md Yasin2 and Seri Narti Edayu Sarchio3 1 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia 2 Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Malaysia 3 Department of Biomedical Sciences, Faculty of Medicine and Health sciences, University of Putra Malaysia, Malaysia Background Since its first discovery, graphene oxide (GO) has emerged as a new carbon-based nanoscale particles due to its extraordinary properties such as high dispersibility, hydrophilicity, large surface area and low production cost. The biological application of GO is however plagued by its poor physical and chemical instability, with a significant toxicity profile in biological milieu. The functionalization of GO with surfactants and polymers are necessary for enhanced stability and biocompatibility. Often, the functionalization of GO involves a complicated series of reactions, organic solvents and harsh synthesis condition, which could limit the yield, increase production cost and aggravate its biocompatibility. The present study aims to develop a simple and cost-efficient, yet stable GO functionalized with Pluronic F127 by means of physical mixing to further advance the potential of GO in biological applications. Methods GO functionalized Pluronic F127 (PF-GO) was synthesized by physical mixing of GO and Pluronic F127 at different ratios. This method was preferred for its simplicity, low cost, yield of small nanosized structure and potential for scale up production. The PF-GO dispersion was characterized for size, size distribution, zeta potential, morphology, chemical characteristics and Pluronic content. The stability of the formulation in physiologically relevant media was also investigated. Results PF-GO showed a discrete structure with a mean size of 235.9 ± 4.2 nm and a narrow size distribution. The functionalization of GO was successfully observed with the increased zeta potential value of -48.3 ± 0.5 nm to -3.79 ± 0.23 nm, and by the FTIR and UV-Vis analyzes. The loading efficiency of Pluronic F127 was found to be 89 ± 5.6 % with batch to batch consistency. The PF-GO was also stable upon storage at room temperature and dispersion with physiologically relevant media. Conclusion The collective data demonstrated the effective stabilization of GO with Pluronic F127 copolymers, which could result in a greater potential for PF-GO as nanocarrier for biological applications. Acknowledgements The research is supported by grant from the Universiti Putra Malaysia (GP-IPM/2016/9513200). The authors acknowledge the facilities, scientific and technical assistance of the Institute of Advanced Technology (ITMA), Universiti Putra Malaysia. Keywords: graphene oxide, Pluronic F127, stability, nanocarriers, characterization Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Miscellaneous Citation: Perumal D, Shamsi S, Md Yasin F and Sarchio S (2019). Characterization of Nano-graphene oxide stabilized by Pluronic as a nanocarrier. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00051 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Suhaili Shamsi, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia, sh_suhaili@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Dharshini Perumal Suhaili Shamsi Faizah Md Yasin Seri Narti Edayu Sarchio Google Dharshini Perumal Suhaili Shamsi Faizah Md Yasin Seri Narti Edayu Sarchio Google Scholar Dharshini Perumal Suhaili Shamsi Faizah Md Yasin Seri Narti Edayu Sarchio PubMed Dharshini Perumal Suhaili Shamsi Faizah Md Yasin Seri Narti Edayu Sarchio Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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