Abstract
Bacteriophage, a predator virus of bacteria, is an abundant biological entity in the biosphere. With ultimate applications in medicine and biotechnology, new phages are extensively being isolated and characterized. The objective of the present study was to characterize lytic bacteriophage vB_Pae-PA14 infecting Pseudomonas aeruginosa ATCC 27853 that was isolated from seawater in Thailand. vB_Pae-PA14 was subjected to whole genome phylogenetic analysis, host range test, biofilm test and characterization. Results showed that the phage belonged to a group of N4-like viruses, could infect P. aeruginosa isolates including carbapenem-resistant P. aeruginosa. The burst size of vB_Pae-PA14 was 86 plaque-forming unit/infected cells. Also, the phage showed a greater ability to control planktonic P. aeruginosa cells than the biofilm cells. Phage could withstand physical stresses especially the high salt concentration. In brief, lytic bacteriophage vB_Pae-PA14 infecting P. aeruginosa was isolated and characterized, which might be useful in further bacteriophage lytic applications.
Highlights
Pseudomonas aeruginosa, a Gramnegative, aerobic bacteria are part of the normal flora of soil and water sources
Pseudomonas phage vB_Pae-PA14 isolation and its morphological analysis To isolate seawater bacteriophage, water was sampled from Chonburi province, Thailand
Sixteen bacterial strains in their log phase were embedded on double-layer agar (DLA) before 5 μl of phage suspensions were spotted
Summary
Pseudomonas aeruginosa, a Gramnegative, aerobic bacteria are part of the normal flora of soil and water sources. It can cause infection in an immunocompromised patient due to broad-spectrum antibiotic resistance and diverse virulence factors including biofilm.[1,2] It contaminates and causes spoilage in food.[3,4] These problems are a global issue limited to developing countries. Drugs and chemical sanitizers are mainly used to control P. aeruginosa.[5] Alternatively, biological control of foodborne pathogens using bacteriophage has been evaluated.[6] multidrug-resistant P. aeruginosa, especially carbapenem-resistant P. aeruginosa (CRPA) is one of the major issues to be a concern worldwide.[7] Recently in the novel COVID-19 pandemic which antibiotics are highly likely overused to protect the patient, the forthcoming emergence of antibiotic resistant bacteria is inevitable.[8] alternative treatment or preventive options are urgently required, with lytic bacteriophages being one of the possible candidates
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