Characterization of microsatellite loci in Sinilabeo rendahli and cross-amplification in four other Chinese cyprinid species

Abstract

Twenty-eight microsatellite loci were isolated in Sinilabeo rendahli, an endemic cyprinid fish in China. In a test on a population of 48 individuals, 15 primer pairs exhibited polymorphism with 2–12 alleles per locus, while the rest 13 were monomorphic. Observed and expected heterozygosities ranged from 0.104 to 0.915 and 0.195 to 0.865, respectively. Four loci showed significant deviation from Hardy–Weinberg equilibrium and two of them had evidence of null alleles. Twenty-six markers successfully cross-amplified in at least one of the four additional Chinese cyprinid species (Garra pingi pingi, Pseudogyrincheilus procheilus, Discogobio yunnanensis and Onychostoma sima) examined in this study.