Characterization of IL-2-dependent cytotoxic T-cell clones: III. Inhibition of killing activity by monosaccharides

Abstract

The effects of monosaccharides on the cytotoxic activity of cytotoxic T lymphocytes (CTL) and three cloned long-term cytotoxic T-lymphocyte lines (CTLL) are compared. Uncultured CTL and clones CTLL-A2 and CTLL-A11 were derived from the peritoneal cavity of C57BL/6 mice immunized against the H-2D d determinants on the BALB/c sarcoma Meth A. Clone CTLL-R5 was derived from spleen of (BALB/c × C57BL/6)F 1 mice immunized against a unique determinant on the BALB/c radiation-induced leukemia RL♂1. The cell-surface phenotype of the clones is Lyt-1 +,2 +,3 +. Cytotoxic activity of CTLL-A2 and CTLL-R5 as determined by a 4-hr 51Cr-release assay was inhibited over 50% by 1 m M 2-deoxy- d-glucose. CTLL-A11 and the uncultured cytotoxic T cells were more resistant to inhibition by 2DG (40% at 20 m M). Surprisingly, it was found that the addition of d-mannose, d-galactose, d-glucose, l-fucose, α-methyl- d-mannose, and N-acetyl- d-glucosamine also inhibited, in a dose-related manner, the cytotoxicity of CTLL-A2 and CTLL-A11. CTLL-R5 showed a more restricted inhibition pattern: only d-mannose and d-galactose were inhibitory. The mechanism of inhibition remains to be clarified.