Abstract
Recently we reported there were at least four types of G O or G O-like proteins in bovine brain membranes based on their elution profiles from Mono Q columns and their immunological reactivities; one of the proteins was purified as an α-monomeric form, and the others as αβγ-trimers. The four proteins, of which α-subunits were confirmed to be a family of G O-type by an immunoblot analysis, were thus referred toas α O 1, G O 2, G O 3 and G O 4, respectively, in order of their elutions from the column. Immunostained peptide mappings arising from proteolytic digestions of the four α-subunits, together with their fragmentation patterns containing radiolabeled ADP-ribose that had been incorporated by pertussis toxin-catalyzed ADP-ribosylation, suggested that the four G O-α were classified into either of two groups such as α 1 and G O 2-α or G O 3-α and G O 4-α. The kinetic parameters of their GTPase activities, however, revealed that there were different properties between α O 1 and G O 2-α or G O 3-α and G O 4-α. Thus, the four G O-type proteins appeared to be different entities from one another.
Published Version
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