Abstract
The leaves of Myrcia DC. ex Guill species are used in traditional medicine and are also exploited commercially as herbal drugs for the treatment of diabetes mellitus. The present work aimed to assess the qualitative and quantitative profiles of M. bella hydroalcoholic extract, due to these uses, since the existing legislation in Brazil determines that a standard method must be developed in order to be used for quality control of raw plant materials. The current study identified eleven known flavonoid-O-glycosides and six acylated flavonoid derivatives of myricetin and quercetin, together with two kaempferol glycosides and phenolic acids such as caffeic acid, ethil galate, gallic acid and quinic acid. In total, 24 constituents were characterized, by means of extensive preparative chromatographic analyses, along with MS and NMR techniques. An HPLC-PAD-ESI-IT-MS and FIA-ESI-IT-MSn method were developed for rapid identification of acylated flavonoids, flavonoid-O-glycosides derivatives of myricetin and quercetin and phenolic acids in the hydroalcoholic M. bella leaves extract. The FIA-ESI-IT-MS techinique is a powerful tool for direct and rapid identification of the constituents after isolation and NMR characterization. Thus, it could be used as an initial method for identification of authentic samples concerning quality control of Myrcia spp extracts.
Highlights
Nowadays, the main concerns about natural medicinal products are effectiveness, safety and the quality of the herbal drugs [1,2]
The optimized HPLC-PAD method was validated for the simultaneous analysis of gallic acid and quercetin in terms of linearity, limit of detection, limit of quantification, precision and accuracy
The qualitative and quantitative profiles of the 70% EtOH extract from the leaves of M. bella were analyzed
Summary
The main concerns about natural medicinal products are effectiveness, safety and the quality of the herbal drugs [1,2]. It is essential to identify and measure all the bioactive constituents of medicinal plants in order to ensure the biological research reliability and repeatability as well as to ensure enhancing the quality control over the pharmacological benefits and/or hazardous. Myrcia bella is a common and important species in many savanna fragments, distributed in the state of. The present work aimed to assess the qualitative and quantitative profiles of M. bella hydroalcoholic extract, due to the potential use of the Myrcia species in tradittional medicine and due to its comercial exploration as an herbal drug to be used in the treatment of diabetes mellitus [15,23,24] once the existing legislation in Brazil determines that a standard method must be developed in order to be used for quality controlling raw plant materials [25]
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