Abstract
From various natural sources on skim milk agar plates 51protease producer bacteria wereisolated by primary screening. Secondary screening was done by zone of clearanceformed by cell free broth on gelatin agar plates. From diameter of zone of clearance potent protease best protease producers Vibrio metschnikovii strain Xmb 057 was isolated. It was biochemically characterized by performing some common biochemical tests. Effect of temperature and pH on protease by Vibrio metschnikovii strain Xmb 057 was studied. Optimum temperature and pH was 500C and 9 respectively make it suitable to be used in leather and detergent industry. Protease was tolerant to Ca++ and Mg++ which suggest its activity in detergents used even in hard water.
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