Abstract
Problem statement: Escherichia coli isolates are the most common bacterial agents of salpingitis in commercial layer hens. The objectives of this study were to determine the phylogenetic groups/subgroups and antibiotic resistance of E. coli isolates from salpingitis cases in commercial layer hens farms in southeast of Iran. Approach: One hundred twenty one E. coli isolates from oviducts of layer hens with salpingitis were examined to determine their O-serogroup, phylogenetic group/subgroup and antibiotic resistance pattern. Results: O-serogroup determination test showed that 47 (38.84%) isolates were typeable and belonged to eleven different O serogroups including: O1, O2, O6, O8, O15, O20, O25, O36, O78, O86 and O111 and 71 (58.67%) isolates were O-nontypeable. Three most prevalent serogroup were O78, O2 and O1 respectively. PCR assays showed that the isolates fall into four phylogenetic groups A (41.32%), D (33.88%), B2 (14.87%) and B1 (9.91%). Ninety nine (81.81%) isolates fell into six phylogenetic subgroups including: A0 (17.35%), A1 (23.96%), B22 (5.78%), B23 (9.09%), D1 (13.22%) and D2 (20.66%). All of the E. coli isolates from O78 serogroup belonged to A phylo-group whereas the O2 isolates mostly fell into D group. The maximum antibiotic resistance was against tetracycline (100%) and minimum resistance was against linco-spectin (37.19%). Twenty four isolates (19.83%) were resistant to all of the examined antibiotics. Twenty three different patterns of multiple drug resistance were observed, out of which N-Te-Sxt-Fm- Nfx-C and N-Te-Sxt-Gm-Fm-Nfx-C-Ls was the two most frequent patterns respectively. The resistant isolates were found in all of the phylogenetic groups and or sub groups. Conclusion: There are similarities between salpingitis derived E. coli strains and other avian pathogenic E. coli isolates in phylogroups, O-serogroups and antibio-resistance patterns.
Highlights
Salpingitis is an inflammation of the oviduct
Salpingitis a complex condition of chickens associated with various bacterial agents including Escherichia coli (E. coli), Gallibacterium anatis, Staphylococcus spp., Mannheimia haemolytica, Streptococcus bovis and occasionally Salmonella spp. (Jordan et al, 2005; Neubauer et al, 2009)
Bacterial strains: A total of 121 E. coli isolates were obtained from oviducts of layer hens with salpingitis and/or salpingoperitonitis
Summary
Salpingitis is an inflammation of the oviduct. Spread of bacteria into the body cavity through the compromised oviduct wall leads to concurrent peritonitis, which is termed salpingoperitonitis (Saif et al, 2003). On the other hand E. coli is one of the opportunist pathogen responsible for number of disease conditions such as yolk sac infection, air sac disease, perihepatitis, enteritis, omphalitis, coligranuloma and colibacillosis (Ghanbarpour and Salehi, 2010). Coli (APEC) serovars involved as causative agents of salpingitis in all birds (layers and broiler breeders, turkeys, ducks and geese) that are sexually mature (Pattison et al, 2007). Several routes of infection have been described: ascending fecal contamination from the cloaca, bacterial translocation respiratory tract According to the serogroups involved, pathogenicity for day-old chicks and virulence indicators, the salpingitis isolates were similar to those from cases of chronic respiratory disease (Monroy et al, 2005)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Animal and Veterinary Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
