Abstract
Amniotic membrane is an effective corneal reconstruction material in veterinary surgery. Cryopreserved amniotic membrane is widely used in practice. Properties of cryopreserved canine amniotic membranes are currently not well studied. This study aimed to compare three properties between canine amniotic membranes cryopreserved for 7 days and 30 days, including tensile strength, transparency, and cell viability. After their respective cryopreservation time, stress–strain curves of the cryopreserved membranes’ tensile strength were assessed using a universal testing machine. Both groups produced J-shaped stress–strain curves with statistically comparable parameters, including maximum stress, strain, and Young’s modulus. The percentage of cell viability was observed by trypan blue staining under a light microscope. Membrane transparency was tested with a spectrophotometer. Transparency tests showed high levels of light transmission and low haze, with no statistical difference between groups. Cell viability was statistically lower in the 30-day cryopreserved group. Tensile strength and transparency of cryopreserved CAM were not significantly impeded for up to 30 days. For CAM to be used as an alternative corneal transplant material in veterinary and regenerative medicine, further research on cell biology, biomechanical properties of the membrane, and cell viability should be conducted.
Highlights
Corneal ulceration is a common ocular disease in veterinary ophthalmology that may lead to numerous complications, including blindness
Clinical use of human Amniotic membrane (AM) has been reported in the treatment of cats [13], dogs [14] and horses requiring corneal reconstruction [15]
This study is aimed to investigate and characterize three properties of cryopreserved canine AM (CAM), namely tensile strength, transparency, and cell viability influenced by storage time for 7 and 30 days
Summary
Corneal ulceration is a common ocular disease in veterinary ophthalmology that may lead to numerous complications, including blindness. Corneal grafting [1] is the most effective procedure for aggressive lesions. Many biological materials, such as the conjunctiva [1], small intestinal submucosa [2], urinary bladder submucosa [3], pericardium [4], amniotic membrane [5,6], and cornea [7], have been reported in use as corneal grafts. Clinical use of human AM has been reported in the treatment of cats [13], dogs [14] and horses requiring corneal reconstruction [15]
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