Abstract
A membrane permeation technique is described which permits characterization of complex formation between small molecules. The method is applicable if the components of the complex diffuse across a nonporous membrane at markedly different rates. The procedure may be used (a) to determine the stoichiometry and stability constant of a complex, (b) to verify values of stability constants obtained by other methods, (c) to check assumptions concerning the stoichiometry of a complex, and (d) to determine the degree of complexation of a compound in systems complicated by the existence of two or more simultaneous equilibria, where other methods of determination may fail or be very time consuming. The membrane permeation technique has been applied to the characterization of the complex formation between salicylamide and caffeine at concentrations where appreciable self-association of caffeine occurs.
Published Version
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