Abstract
Tetraspore mucilage and rhizoids of Champia parvula (C. Agardh) Harvey were characterized with histochemical stains and fluorescein isothiocyanate (FITC)-Iabelled lectins and examined by SEM and light microscopy. The extracellular mucilage of attached tetraspores, rhizoids, and holdfasts stained positive for sulphated and carboxylated polysaccharides. Using a variety of FITC-lectins to probe the mucilage of attached tetraspores, we detected the sugar haptens α-d-mannose, α-d-glucose, β-d-galactose, and N-acetylgalactosamine. N-acetylglucosamine and α-l-fucose were not detected with FITC-lectins specific for these sugar haptens. Spores, rhizoids, and holdfast cells stained for protein, whereas their accompanying mucilage did not stain for protein.
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