Abstract
In recent years, endophytic fungi associated with medicinal plants have received much attention because of their special ability to synthesize analogous and non-analogous secondary metabolites with interesting biological activities. This study focuses on antibacterial potential of endophytic Nigrospora oryzae isolated from Vitex negundo, a medicinal plant collected from Melghat forest of Amravati District of Maharashtra, India. The endophytic fungus was grown over Potato Dextrose Broth (PDB) and then further extracted with ethyl acetate (EA). The crude EA extract was further screened for its antibacterial property via disc diffusion method against three pathogenic microorganisms such Escherichia coli (MTCC 1698), Pseudomonas aeruginosa (MTCC 6458) and Staphylococcus aureus (MTCC 2639). The crude EA residue exhibited prominent microbial activity (11.16–16.67 mm) against tested pathogens. Furthermore, the EA extract was analysed by GC/MS for characterization of bioactive fungal metabolites. TheGC/MS analysis suggested the presence of major compounds like phenol 2,4-bis(1,1-dimethylethyl), 3-hexadecyloxycarbonyl-5-(2-hydroxyethyl)-4-methylimidazolium ion, naphthalene, 1,2-benzenedicarboxylic acid diisooctyl ester and eicosene. The antibacterial activity exhibited by the EA extract might be attributed to these compounds solely or synergistically.
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