Abstract
We devised a new murine orofacial infection model using bacteria from odontogenic infection origins and characterized the experimental infections. In this model, bacteria were injected into the submandible of mice. Streptococcus constellatus and Peptostreptococcus micros produced a single abscess at the injection site and their abscess-forming and lethal abilities were low: the median abscess-forming dose (AF50) of S. constellatus and P. micros were 108.5–10.7and 1010.2–10.6cfu/mouse, and their median lethal dose (LD50) were >11 and 1010.6–11cfu/mouse, respectively. Prevotella oralis and Fusobacterium nucleatum produced multiple abscesses and their abscess-forming and lethal abilities were strong: AF50of P. oralis andF. nucleatum were 106.0–6.4and 107.0–8.7cfu/mouse, and their LD50were 107.0–7.7and 108.3–9.9cfu/mouse, respectively. LD50of P. intermedia andP. gingivalis were 109.4–>11and 108.9–9.1cfu/mouse, respectively. Prevotella intermedia and Porphyromonas gingivalis generated a necrotizing lesion, which progressed rapidly. We conclude that this murine model could reflect human orofacial odontogenic infections and is useful to investigate the pathogenicity of causative bacteria of such infections.
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