Abstract
Four T cell hybridoma clones were established and characterized from 250 hybridoma cell lines and characterized. All clones were strongly positive for Thy-1 and H-2 antigens. The Lyt-1 and L3T4 determinants were weakly but significantly positive, whereas I-A antigens and surface immunoglobulin were negative. Three of the clones secreted IL-2 upon stimulation with H-2Kk cells, whereas the remaining clone did not. This IL-2 secretion was stimulated not only by macrophage-dendritic cells, but also by B cells. All clones produced significant footpad swelling following injection into the footpads of H-2k mice. The time course and histology of the footpad swelling suggested that the reaction might be a delayed type hypersensitivity (DTH)-like response.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call