Abstract
Aleutian mink disease virus (ADV), propagated in normal mink kidney cell cultures, was resolved by centrifugation on CsCl density gradients into two components of densities 1.334 and 1.295 gm/cm 3 which were, respectively, about 85 and 15% of the total virus yield. Electron microscopic examination revealed that the major component contained predominantly intact virions of 25 nm in diameter which resembled virus particles with icosahedral structure while the minor component contained mainly empty capsids. Both the empty capsids and intact virus particles absorbed to immunoabsorbents prepared with IgG from chronically infected mink. The buoyant density, size, and structure of purified ADV are in common with viruses belonging to the picornavirus group.
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