Characterization of a planar l-glutamate amperometric biosensor immobilized with a photo-crosslinkable polymer membrane

Abstract

A photo-crosslinkable polymer mixed with bovine serum albumin was adapted to fabricate an amperometric planar l-glutamate biosensor. The characteristics of this amperometric biosensor during aging of the membrane, its reproducibility and the effects of storage conditions on the long-term stability of the biosensor were studied. The steady-state method (R.S.D. = 3.69%) was more appropriate than the kinetic method (R.S.D. = 7.70%) for measuring the concentration monosodium glutamate. The l-glutamate amperometric biosensor exhibited remarkable long-term stability (150 days) when maintained in a dry dark state. Most of the functional characteristics of the biosensor were restored within 5 min (wet-up times) of repeated wetting and drying. The long-term stability and short wet-up times of the electrode suggests its suitability for use in the design of real time or flow injection biosensors.