Abstract
ABSTRACT Oligosaccharides produced by degradation of hyaluronan (HA) can be applied in functional foods. In this study, Variovorax sp. J25 with high secreted HA lyase activity (16.0 U/mL), was isolated from soil, and its properties were investigated. The activities toward chondroitin sulfates A and C (CS-A and CS-C) were much lower, at 0.439 and 0.325 U/mL, respectively. The molecular weight of the purified HA lyase was around 73 kDa. The optimal reaction pH and temperature were approximately 6.7 and 50°C, respectively. The amounts of sugar produced from HA, CS-A, and CS-C were 550, 175, and 271 mg/g after reaction for 24 h, respectively. The degradation of CS-A and CS-C proceeded gradually. The major product from HA was disaccharide [ΔUA–GlcNAc], and tetrasaccharide [(ΔUA–GlcNAc)2] was also detected (GlcNAc: N-acetylglucosamine, ΔUA: 4-deoxy-α-L-threo-hex-4-enepyranosyluronic acid). In the degradation of CS-A and CS-C, disaccharide [ΔUA–GalNAc-OSO3H] was released (GalNAc: N-acetylgalactosamine).
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