Characterization of a Coliphage AS1 isolated from sewage effluent in Pakistan.

A Ullah,Kalsoom Kalsoom,S Ahmad,M Abbas,N Begum,A Sultan,F A Khan,J Jamil,M A K Khattak,S U Din,T Qamash

doi:10.1590/1519-6984.240943

Abstract

The emergence of multi-drug resistant (MDR) bacterial strains, which are posing a global health threat has developed the interest of scientists to use bacteriophages instead of conventional antibiotics therapy. In light of an increased interest in the use of phage as a bacterial control agent, the study aimed to isolate and characterize lytic phages from sewage effluent. During the current study, bacteriophage AS1 was isolated from sewage effluent against E.coli S2. The lytic activity of phageAS1 was limited to E.coli S2 strain showing monovalent behavior. The calculated phage titer was 3.5×109 pfu/ml. PhageAS1 was stable at a wide range of pH and temperature. The maximum stability was recorded at 37ºC and pH 7.0, while showing its normal lytic activity at temperature 60ºC and from pH 5.0 to11.0 respectively. At temperature 70ºC, phage activity was somewhat reduced whereas, further increase in temperature and decrease or increase in pH completely inactivated the phage. From the current study, it was concluded that waste water is a best source for finding bacteriophages against multi-drug resistant bacterial strains and can be used as bacterial control agent.

Highlights

The emergence of multi-drug resistant (MDR) bacterial strains, which are posing a global health threat, has developed the interest of scientists to use bacteriophages instead of conventional antibiotics therapy
E.coli is a gram negative, rod shape and non-spore forming bacteria and a member of normal flora of human gastrointestinal tract, where it is present abundantly and helps in maintaining the normal physiology of their host (Levinson, 2014).Based on pathogenicity, E.coli is divided into two categories
300ml sewage water was collected in a sterile bottle from different localities of district Swabi KPK Pakistan from December 2017 to April 2018

Summary

Introduction

The emergence of multi-drug resistant (MDR) bacterial strains, which are posing a global health threat, has developed the interest of scientists to use bacteriophages instead of conventional antibiotics therapy. Using different mechanisms the pathotypes of Enteric E.coli can cause different types of gastrointestinal diseases and sometimes can cause disease outside the intestine (Clements et al, 2012).The pathotypes Uropathogenic of Extraintestinal E.coli is the most common cause of nosocomial UTI infection. The infection may lead to kidney failure due to which E.coli is considered as the most common pathogen responsible for kidney failure. E.coli strain make biofilm on the surface of different medical devices which is pertaining to different nosocomial infections (Surette et al, 1999; Naves et al, 2010; Choisy, 2011)

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