Abstract
Integration of biology with nanotechnology is now becoming attention-grabbing area of research. The antimicrobial potency of silver has been eminent from antiquity. Due to the recent desire for the enhancement of antibacterial efficacy of silver, various synthesis methods of silver in their nano dimensions are being practiced using a range of capping material. The present work highlights a facile biomimetic approach for production of silver nanoparticle being capped and stabilized by putrescine, possessing a diameter of 10–25 ± 1.5 nm. The synthesized nanoparticles have been analyzed spectrally and analytically. Morphological studies are carried out by high-resolution transmission electron microscopy and crystallinity by selected area electron diffraction patterns. Moreover, the elemental composition of the capped nanoparticles was confirmed by energy-dispersive X-ray spectroscopy analysis. A comparative study (zone of inhibition and minimum inhibitory concentration) regarding the interactions and antibacterial potentiality of the capped silver nanoparticles with respect to the bare ones reveal the efficiency of the capped one over the bare one. The bacterial kinetic study was executed to monitor the interference of nanoparticles with bacterial growth rate. The results also highlight the efficacy of putrescine-capped silver nanoparticles as effective growth inhibitors against multi-drug resistant human pathogenic bacterial strains, which may, thus, potentially be applicable as an effective antibacterial control system to fight diseases.
Highlights
Nanomaterials are the keystone of the nanoscience and nanotechnology research
To ascertain the synergistic effect of silver nanoparticles along with plant natural polyamines against bacteria, in the present work, we explore a biomimetic capping procedure that permits us to stabilize silver nanoparticles with plant natural polyamine, attributing polycationic moieties to investigate their successful usage as antibacterial agents against some pathogenic bacterial strains of public concern
As observed from high-resolution transmission electron microscopy (HRTEM) images, the synthesized capped nanoparticles were more or less spherical, well dispersed, uniform and attained a hydrodynamic diameter of 10–25 ± 1.5 nm (Fig. 3a) which was confirmed by dynamic light scattering (DLS) measurements (Fig. 3d)
Summary
Nanomaterials are the keystone of the nanoscience and nanotechnology research. The knowledge of merging nanomaterials in the area of biology and its applications is potentially revolutionizing in many fields of science and technology. The preferential use of spherical silver nanoparticles over its other shapes, as an antimicrobial agent, is due to their high reactivity for their large surface area to volume ratio (Roduner 2006). Recently silver nanoparticles have been studied as biomarkers (Jain et al 2008; Schoefield et al 2006) for their localized surface plasmon resonance. They are mostly popular for their antimicrobial applications like, wound dressings (Tian et al 2007), water treatment (Lv et al 2009), fabrics and textile processing (Vo Ke Thanh and Phuong Phong 2009) and more recently in agronomy (Mandeh et al 2012; Abdi et al 2008; Saha et al 2015)
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