Abstract
We analyzed 11 samples of wastewater, sludge and soil contaminated with acrylic acid nitrile, acrylamide and acrylic acid from the territory of Navoiyazot JSC (Navoi, Uzbekistan). As a result, 19 strains belonging to the bacteria of the genus Rhodococcus were isolated into a pure culture. In the process of selection, 2 strains of bacteria were selected that exhibit the highest amidase activity for acrylic acid (0.712 U / mg and 0.868 U / mg). Strains of Rhodococcus sp. - 8/4/1 and Rhodococcus sp. - 3/4/3 are potential amidase producers.
Highlights
Rhodococci possess a high level of adaptation to extreme conditions of existence [1,2], are characterized by such unique biological properties as pleomorphism, the ability to coaggregate, have a complex morphogeneticThe USA Journals Volume 03 Issue 12-2021 development cycle, and these characteristics determine the presence of various methods of cell cooperation [3]
To isolate active strains of microorganisms capable of transforming nitriles, samples of wastewater, silt and soil contaminated with NAC, AA and Acrylic acid were analyzed
Individual colonies of microorganisms were isolated by the method of enrichment culture and direct seeding
Summary
Rhodococci possess a high level of adaptation to extreme conditions of existence [1,2], are characterized by such unique biological properties as pleomorphism, the ability to coaggregate, have a complex morphogeneticThe USA Journals Volume 03 Issue 12-2021 development cycle, and these characteristics determine the presence of various methods of cell cooperation [3]. The aim of this work is to isolate local strains of bacteria - producers of nitrile transforming enzymes, in particular amidases, and to assess the possibility of their use for obtaining acrylic acid. To isolate microorganisms with amidase activity, wastewater, sludge and soil contaminated with nitrile acrylic acid, acrylamide and acrylic acid JSC “Navoiyazot” (Navoi, Uzbekistan) were used.
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