Abstract
Polyculture of microalgae could enhance biomass production. It is crucial to select the proper combination of microbial polyculture which can achieve a positive impact. Ten microalgae suitable for healthy growth in municipal wastewater were selected. Simulated wastewater was used to conduct experiments on the cultivation of single microalgae. Possible combinations of microalgae were analyzed from three aspects: the potential for conversion into biofuels, the consumption of different forms of nitrogen and phosphorus, and the structure of microalgae bacterial communities. From the perspective of converting to biocrude, Leptolyngbya boryana with high protein content was found unsuitable as a biomass raw material. Non-metric multidimensional scale analysis of different forms of nitrogen and phosphorus consumption shows the preference of the microalgae community for the use of nitrogen and phosphorus. By analyzing the bacterial community structure, it was found that microalgae have a significant impact on the bacterial community. We believe that it is more likely to improve the production efficiency of microalgae by establishing the combination of microalgae with high biocrude conversion efficiency, different nitrogen and phosphorus utilization preferences, and large difference in bacterial community structure.
Highlights
A diverse energy system has been established in the world, primarily including fossil energy, nuclear energy, renewable energy, and hydro-energy
Methanol was added to the centrifuge tube for vortex oscillation; (3) 10 mL methyl tert-butyl ether (MTBE) was added and the reagent was cultivated on a shaking table at 25 ◦ C for 1 h; (4) 2.5 mL water was added to induce phase separation; (5) After incubation at 25 ◦ C for 10 min, the upper organic phase was collected after the sample was centrifuged at 1000× g for 10 min; (6) the organic phase is transferred in a weighed tin tray, which was placed in the clean fume hood; and (7) after the organic solvent is completely volatilized, the tin tray is transferred to the oven and dried at 80 ◦ C to constant weight
Leptolyngbya boryana is better than Micractinium sp. in maximum optical density and growth rate, but Micractinium sp. is more stable and less different than Leptolyngbya boryana, which may indicate that
Summary
A diverse energy system has been established in the world, primarily including fossil energy, nuclear energy, renewable energy, and hydro-energy. Microalgae may be the most potential biomass raw material [2]. The high productivity potential of microalgae is one of the main driving factors for its use as a raw material for biofuel production. There are still many problems in the commercial application of using microalgae as biomass raw materials. The density of outdoor cultivation of microalgae is too low, steady biomass production at a large scale in outdoor venues is difficult, high biomass yield and high lipid content are usually contradictory, the cost of harvesting microalgae is too expensive, etc. To turn microalgae biofuels into a commercially feasible method, the microalgae cultivation system needs to have the following characteristics: high biomass yield, high biomass quality and stable operation [8]. Cost reduction is the key to the commercialization of biofuels and using municipal wastewater as a culture medium is very helpful in this regard [9]
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