Abstract
The aim of this work was to characterize spent mushroom substrate polysaccharides (MSP) from Pleurotus eryngii and their antioxidant and organ protective effects in streptozotocin- (STZ-) induced diabetic mice. The enzymatic-, acidic-, and alkalic- (En-, Ac-, and Al-) MSP were extracted from P. eryngii with snailase (4%), hydrochloric acid (1 mol/l), and sodium hydroxide (1 mol/l), respectively. The characterizations were evaluated by spectral analysis. In animal experiments, the enzymatic activities, lipid peroxide contents, and serum lipid parameters were measured, and histological observations of the liver, kidney, pancreas, and heart were conducted. The results demonstrated that treatment with En-, Ac-, and Al-MSP increased the organ enzymatic activities, decreased the organ lipid peroxide contents, mitigated the serum biochemistry values, and ameliorated the histopathology of diabetic mice, indicating that En-, Ac-, and Al-MSP could potentially be used as functional foods for the prevention of diabetes.
Highlights
Diabetes mellitus (DM), an endocrine metabolic disease characterized by a group of complex, chronic symptoms, is usually considered the major health risk worldwide due to its stimulation of health complications including heart disease, blindness, and organ failure [1, 2]
The highperformance liquid chromatography (HPLC) chromatograms indicated that the weight average molecular weight (Mw), number average molecular weight (Mn), and Z-average molecular weight (Mz) of En-mushroom substrate polysaccharides (MSP) were 1.35 × 103, 1.21 × 103, and 1.33 × 103 Da; the Mw, Mn, and Mz of Ac-MSP were 2.11 × 103, 2.54 × 103, and 2.19 × 103 Da; and the Mw, Mn, and Mz of Al-MSP were 2.26 × 103, 2.85 × 103, and 1.93 × 103 Da, respectively
The monosaccharide composition of En, Ac, and Al-MSP was analyzed according to the retention time of standard monosaccharides (Figure 1(a))
Summary
Diabetes mellitus (DM), an endocrine metabolic disease characterized by a group of complex, chronic symptoms, is usually considered the major health risk worldwide due to its stimulation of health complications including heart disease, blindness, and organ failure [1, 2]. It has been reported that approximately 552 million people will suffer from DM by the year 2030 [3]. Several studies have reported that the occurrence and progression of DM and its complications involve many factors, including hemodynamic disorders, genetic predisposition, and disorders of biochemical metabolism [4]. It is vitally important to seek natural and nontoxic antioxidants to prevent and treat DM. The focus has recently shifted to identifying harmless natural antioxidants from edible materials [7]
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