Abstract
A water-soluble polysaccharide (OJP2) obtained from the roots of Ophiopogon japonicas, was precipitated with 95% ethanol and purified by DEAE-52 cellulose anion-exchange and Sephadex G-100 gel filtration chromatography. The characteristics of OJP2 were determined by chemical analysis, high performance gel permeation chromatography (HPGPC), and gas chromatography-mass spectrometry (GC-MS). The results showed that the average molecular weight (Mw) of OJP2 was 35.2 kDa, and five kinds of monosaccharides including rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 0.5:5:4:1:10. Furthermore, the antioxidant activity of OJP2 was evaluated in H2O2-treated HaCaT cells and glucose-treated LO2 cells. The results show that OJP2 can increase the activity of SOD and NO production, and decrease the level of MDA in these two kinds of injury cells. OJP2 should be explored as a novel and potential natural antioxidant agent for use in functional foods or medicine.
Highlights
Free radicals, the highly reactive molecules, play an important positive physiological role and, at the same time, they may exert toxic effects [1] [2]
The polysaccharides extracted from the root of O. japonicus were purified through with DEAE-cellulose column and Sephadex G-100 column and named OJP2
The average molecular weight of OJP2 was determined as 88.1 kDa by high performance gel permeation chromatography (HPGPC)
Summary
The highly reactive molecules, play an important positive physiological role and, at the same time, they may exert toxic effects [1] [2]. There is increasing evidence that free radicals are able to damage cell membranes and numerous biological substances [3] [4], resulting in various diseases including cardiovascular diseases, cancer, aging, Parkinson’s and Alzheimer’s disease, atherosclerosis, and impairment of immune function [5]-[8]. Antioxidants can scavenge free radicals and help to reduce oxidative damage. Polysaccharides extracted from natural sources have been found to have variety of biological activities, such as antioxidant, immunobiological and antitumor activity, which have attracted lots of attention in the biochemical and medical area [9]-[13]. The polysaccharides isolated from the roots of O. japonicus have drawn the attention of researchers and consumers due to their nutritional and health protective value in hypoglycemic, anti-ischaemia, immunostimulation, inhibiting platelets aggregation, etc. The structure and function of these polysaccharides have not been well characterized
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