Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae

Xiaoli Wang,Mingqin Zhao,Yifei Zhang,Zhikai Liu,Pengfei Liu

doi:10.3390/molecules22111866

Abstract

In this study, crude Cortex Periplocae polysaccharides (CCPPs) were extracted with water. CCPPs were decolored with AB-8 resin and deproteinated using papain-Sevage methods. Then, they were further purified and separated through DEAE-52 anion exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Three main fractions—CPP1, CPP2, and CPP3, (CPPs)—were obtained. The average molecular weights, monosaccharide analysis, surface morphology, and chemical compositions of the CPPs were investigated by high-performance gel permeation chromatography (HPGPC), gas chromatography-mass spectrometry (GC/MS), UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectrum, and nuclear magnetic resonance (NMR). In addition, the antioxidant activities of these three polysaccharides were investigated. The results indicated that all of the CPPs were composed of rhamnose, arabinose, mannose, glucose, and galactose. These three polysaccharides exhibited antioxidant activities in four assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2′-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical, reducing power, and total antioxidant activity in vitro. The data indicated that these three polysaccharides could be utilized as potential natural sources of alternative additives in the functional food, cosmetics, and pharmaceutical industries.

Highlights

In recent years, due to the development of new lifestyles and work pressure, there has been an increasing demand for functional foods, such as polyphenols, phospholipids, chitins, etc.To date, polysaccharides have been widely studied and found to be one of the most important biological macromolecules in Nature because of their wide range of pharmacological activities, such as antioxidant, antityrosinase, antitumor, antihypertensive, immune-enhancement, and many others [1,2,3,4,5,6,7]
Glc and Gal; CPP2 was rich in Rha, Ara, Glc, and Gal; and CPP3 was rich in Rha, Glc, and Gal
The analysis showed that each around 4.4 ppm showed that these three polysaccharides contained a small amount of β-glycosidic of the three polysaccharides consisted of different monosaccharides, which meant that they were bonds

Summary

Introduction

Due to the development of new lifestyles and work pressure, there has been an increasing demand for functional foods, such as polyphenols, phospholipids, chitins, etc.To date, polysaccharides have been widely studied and found to be one of the most important biological macromolecules in Nature because of their wide range of pharmacological activities, such as antioxidant, antityrosinase, antitumor, antihypertensive, immune-enhancement, and many others [1,2,3,4,5,6,7]. Due to the development of new lifestyles and work pressure, there has been an increasing demand for functional foods, such as polyphenols, phospholipids, chitins, etc. In China, polysaccharides have been developed into medicines, for example, Maitake Polysaccharides Capsule, Astragalus Polysaccharide Injection, Lentinan Injection, and Polysaccharides of G. Polysaccharides have good water-holding capacity, fat-binding ability, emulsifying property, thermal stability, and sustained-release property, so they can be used in cosmetics, pharmaceuticals, food, and other products in various fields [7,9,10,11,12,13]. More and more researchers are increasingly interested in discovering new polysaccharides from various sources

Methods

Results

Conclusion