Abstract
Abstract: The Canna Striata fish is a potent source of albumin as an antioxidant. Albumin is a protein capable of binding to free radicals in plasma and has 17 disulfide bonds linking sulfur-containing amino acids. The existence of thiol group bonds in albumin extract of Canna Striata fish is possible to bind to diphenylphycril hydrazyl (DPPH), causing the antioxidant capacity of Canna Striata fish extract to be high. The accumulation of free radicals produced by the administration of antituberculosis drugs from the results of isoniazid metabolism can cause hepatic necrosis. So it requires antioxidants that can reduce free radical levels, which in this study was tested in white rats induced with isoniazid and then treated with albumin protein extract Canna Striata fish. This research method is the first experimental method done is the characterization of albumin protein extract Canna Striata fish with temperature 25˚C and 40˚C by Sodium Dodecyl Sulfate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Then the antioxidant level was tested by measuring the levels of Malondialdehyde (MDA) after 2 last week treatment period in 5 groups of white rats are positive control group, negative control group and 3 treatment groups induced Isoniazid toxic dose are 37.8 mg accompanied by giving of albumin protein extract Canna Striata fish with content of 20ml/kg, 40 ml/kg and 60 ml/kg. The result of this study showed that there were ten albumin protein profiles with size 15,41 kDa, 19,74 kDa 28,6 kDa, 38,87 kDa, 46,08 kDa, 72,45 kDa, 94,42 kDa , 120.82 kDa, 142.80 kDa, and 155.97 kDa. Based on One Way ANOVA and Post Hoc Tukey statistic test, it were found that there was the significant influence and differentiation of Malondialdehyde (MDA) level on giving of albumin protein extract in white rats induced by Isoniazid with p-value 0,002. The most effective decrease in MDA levels is high levels of albumin protein extract of 60 ml/kg. Keywords: Albumin Protein Extract of Canna Striata, Malondialdehyde, Isoniazid
Highlights
Global tuberculosis control in the WHO in 2009 reported that in Indonesia, Pulmonary TB became a major public health topic
Based on the description above, the authors are interested to examine the characterization of albumin protein profile in Canna Striata fish and its potential as an antioxidant in white rats induced by isoniazid toxic dose
To see whether there was any effect of the extract of Channa striata on the level of Malondialdehyde (MDA) on white rats (Rattus novergicus) induced by Isoniazid (INH) performed by One Way ANOVA test
Summary
Global tuberculosis control in the WHO in 2009 reported that in Indonesia, Pulmonary TB became a major public health topic. Santoso et al, (2009) reported in his research that administration of Canna striata fish extracts could withstand a decrease in serum an antioxidant activity due to paracetamol poisoning. This albumin is due to the high activity of antioxidants due to the presence of albumin and minerals in Canna striata fish extract. Canna striata fish contains compounds - compounds that are important for the human body such as high enough protein, fat, water and minerals. Based on the description above, the authors are interested to examine the characterization of albumin protein profile in Canna Striata fish and its potential as an antioxidant in white rats induced by isoniazid toxic dose. The following are table which shows levels of Malondyaldehyd on the treatment Rat white
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