THE MEASUREMENT OF MALONDIALDEHYDE LEVEL IN HEART TISSUE OF HYPERLIPIDEMIC RATS AFTER INTERVENTION OF COMBINATION OF JICAMA (PACHYRHIZUS EROSUS) CONCENTRATE AND KEFIR MILK AS A SYN

Abstract

Abstract Objective: This research aims to know the effect of synbiotic drink from jicama concentrate and kefir milk on malondialdehyde (MDA) level in heart tissue of hyperlipidemic rats. Design and method: Twenty five rats divided into 5 groups such as negative control (K-), positive control (K+), and intervention groups (P1, P2, and P3). All animal model was acclimatized for seven days. Group of K+, P1, P2, and P3 were given quail egg yolks 5 ml/200 grBW/day for 4 weeks to make the hyperlipidemic model. For next 4 weeks, the intervention groups (P1, P2, and P3) were given a different formulations synbiotic drink with 5 ml/ 200 grBW/day. There are three formulations of synbiotic drink, P1: 85% Kefir Milk (KM), 15% Jicama Concentrate (JC), P2: 75% (KM), 25% (JC), P3: 65% (KM), 35% (JC). In the end of experiment, the animal model was terminated to get the heart organs. The heart organs were saved in a cup which have filled with alcohol and began to measure MDA level in heart tissue. All data were statistically analyzed with One-Way Annova. Values were considered significant at p < 0,05. Results: Based on data, all of the data were expressed as mean ± SD. The mean of MDA level (nmol/gr) in negative control group (K-) was 1.50 ± 0.08 and positive control group (K+) was 10.70 ± 0.14. The groups of intervention were 6.60 ± 0.09 (P1), 4.56 ± 0.11 (P2), and 3.02 ± 0.13 (P3). The result showed there was significant differences between all groups (p < 0,001). Conclusions: Synbiotic drink from jicama concentrate and kefir milk significantly to improve MDA level in heart tissue of hyperlipidemic rats and can be considered for adjuvant therapy to decrease the risk of hypertension.