Abstract
The objectives of the present study were the investigation of antioxidant activity of oil extract of Araucaria cunninghamii Aiton ex D. Don and identification of classes of antioxidants with HPLC analysis. The antioxidant potential of the oil extract of Araucaria cunninghamii Aiton ex D. Don was evaluated by DPPH assay, reducing power assay and nitric oxide scavenging assay. Total antioxidant capacity, total phenolic and flavonoid content were also calculated. The oil extract of Araucaria cunninghamii Aiton ex D. Don was investigated for polyphenols qualitatively as well as quantitatively using High-performance liquid chromatography (HPLC). Oil extract of Araucaria cunninghamii Aiton ex D. Don showed antioxidant activity in different assays in a dose dependent manner. A positive correlation between the antioxidant activity and phenolic content was observed. HPLC studies revealed the presence of Gallic acid, catechin, chlorogenic acid, epicatechin, caffeic acid, umbelliferone, rutin, ellagic acid, quercetin, kaempferol in the oil extract. Results indicate that the oil extract of Araucaria cunninghamii Aiton ex D. Don possess antioxidant activities which can be correlated with the polyphenolic compounds identified by HPLC analysis.
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