Characteristics of gel chromatography using Sephadex gel for fractionation of soluble organic pollutants

Abstract

The elution volumes of 46 soluble organic compounds in gel chromatography using Sephadex G-15 and G-25 were measured, and the fractionation of organic pollutants by gel chromatography was discussed. In the case of compounds whose structures consist of some unit structure like polyethyleneglycols and sugars, the elution volume increased with the decrease of molecular weight as expected. But for many low molecular weight compounds, this conventional relationship did not apply. The relations between the elution volume and the chemical structure of organic compounds were also discussed.