Elution behaviour of low molecular weight compounds in size exclusion chromatography

Abstract

Research Article| February 01 2012 Elution behaviour of low molecular weight compounds in size exclusion chromatography Aki Sebastian Ruhl; Aki Sebastian Ruhl 1Berlin Institute of Technology, Chair of Water Quality Control, Str. des 17. Juni 135, 10623 Berlin, Germany E-mail: aki.s.ruhl@tu-berlin.de Search for other works by this author on: This Site PubMed Google Scholar Martin Jekel Martin Jekel 1Berlin Institute of Technology, Chair of Water Quality Control, Str. des 17. Juni 135, 10623 Berlin, Germany Search for other works by this author on: This Site PubMed Google Scholar Journal of Water Supply: Research and Technology-Aqua (2012) 61 (1): 32–40. https://doi.org/10.2166/aqua.2012.138 Article history Received: May 19 2011 Accepted: November 15 2011 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Cite Icon Cite Permissions Search Site Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsThis Journal Search Advanced Search Citation Aki Sebastian Ruhl, Martin Jekel; Elution behaviour of low molecular weight compounds in size exclusion chromatography. Journal of Water Supply: Research and Technology-Aqua 1 February 2012; 61 (1): 32–40. doi: https://doi.org/10.2166/aqua.2012.138 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex Size exclusion chromatography (SEC) in combination with continuous organic carbon detection (OCD) is a powerful analytical technique that enables characterization of dissolved organic water constituents. Low molecular weight organic water constituents are ubiquitous but their behaviour in SEC analyses is not yet fully clarified. Therefore, we analysed a number of low molecular weight organic model compounds with various structural and functional characteristics by size exclusion chromatography combined with online OCD and ultraviolet light absorption measurement (UVD). The detection times of some low molecular weight organic compounds were much lower than expected. Elution behaviour of low molecular weight compounds (below 300 g/mol) was determined by functional groups rather than by molecular weight. Retention times of low molecular weight compounds varied between 51.9 min, close to that of humic substances, and 235 min of benzaldehyde, the double value of a standard chromatogram runtime of 120 min. Comparisons of both detectors' signals reveal that elution times and detection times are not identical. The elution sequence of various model compounds provided here facilitates identification of peaks in the low molecular weight range of SEC chromatograms. humic substance, low molecular weight compound, NOM, organic carbon detection, size-exclusion chromatography This content is only available as a PDF. © IWA Publishing 2012 You do not currently have access to this content.