Abstract
IntroductionThis study was aimed to evaluate the prevalence of diarrheagenic Escherichia coli (DEC) and ESBL genes in the children with gastroenteritis and to explore the predominant pathotypes of DEC with the multiplex PCR (MPCR) type. MethodsTo investigate the prevalence of DEC in the young children, 303 samples were obtained from children with gastroenteritis and evaluated by the MPCR test to characterize the five pathotypes of DEC. ResultsDEC infections were found in 68 cases (22.44%). Enteroaggregative E. coli (EAEC) was the most prevalent pathotype (80.9%, 55/68) determined by three primers sets. Detection rate for Enteropathogenic E. coli (EPEC), Enterotoxigenic E. coli (ETEC), Enteroinvasive E. coli (EIEC) and Shiga toxin producing E. coli (STEC) was 8.8% (6/68), 8.8% (6/68), 4.4% (3/68) and 4.4% (3/68), respectively. The frequency DEC was not statistically significant with age and the gender of the patients (P ˃0.05). The blaSHV gene was not found in the isolates and the blaTEM and blaCTX-M genes were identified in 29.4% and 25% of the isolates, respectively. ConclusionsDEC could be regarded as one of the major causative agents of children diarrhea, thus emphasizing the role of EAEC as the main causative pathotype of DECs. Detection of a high number of ESBL genes might be associated to the limited management of antimicrobial resistance among these isolates.
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