Abstract
The content of fish oil supplements is sufficient to fulfill the need of EPA and DHA, however adding nutritional value will be beneficial. This study was aimed to characterize the chemical content of the supplement from a combination of shark liver oil and Spirulina powder. The oil was extracted using dry rendering method at 50oC for 8 hours. The extracted oil was then combined with Spirulina powder with a concentration of 10; 30; and 50%. In general, the chemical characteristics of fish oil were affected by the addition of Spirulina plantesis. The fish oil with adddition of Spirulina 50% had the highest protein (24.3%) and essential amino acid content (but comparable DHA content. In conclusion, addition of Spirulina increased the chemical content of the fish oil, but reduced their fatty acid content.
Highlights
The content of fish oil supplements is sufficient to fulfill the need of Eikosapentaenoat acid (EPA) and DHA, adding nutritional value will be beneficial
The extracted oil was combined with Spirulina powder with a concentration of 10; 30; and 50%
The chemical characteristics of fish oil were affected by the addition of Spirulina plantesis
Summary
Bahan utama yang digunakan dalam penelitian ini adalah hasil samping ikan cucut berupa hati ikan cucut dan mikroalga Spirulina. Meliputi NaOH 0,5 N dalam metanol, BF3 20%, NaCl, isooctane, dan Na2SO4 anhidrat, KOH 0,1 N, alkohol netral 96%, indikator penolftalein, asam asetat, kloroform, akuades, natriumthiosulfat 0,02 N dan kalium iodin. Alat utama dalam proses ekstraksi asam lemak omega-3 minyak ikan adalah blender (miyako CH-501, China), heating drying oven (DHG-9053A, Japan), sentrifuge (Hitachi Model Part No R12A6904357D0, Japan). Alat untuk analisis lemak berupa Gas Chromatography (GC) Shimadzu Model GC 2010 plus dengan kolom cyanopropil methyl sil (capillary column), dimensi kolom p= 60 m, Ø dalam = 0,25 mm, 0,25 μm film thickness, laju alir N2 20 mL/menit, dan laju alir H2 30 mL/menit. Peralatan untuk analisis lainnya antara lain timbangan electronic kitchen scale (SF-400, China), hot plate (Favorit HP0707V2, Malaysia), mikro pipet (Gilson, Swiss), dan UV-Vis RS Spectrophotometer (UV-2500, Japan)
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