Abstract
West Nile virus disease (WND) is an arthropod-borne zoonosis responsible for nonspecific fever or severe encephalitis. The pathogen is West Nile virus belonging to the genus Flavivirus, family Flaviviridae. Every year, thousands of cases were reported, which poses significant public health risk. Here, we constructed a West Nile virus chimera, ChiVax-WN01, by replacing the prMΔE gene of JEV SA14-14-2 with that of the West Nile virus NY99. The ChiVax-WN01 chimera showed clear, different characters compared with that of JEV SA14-14-2 and WNV NY99 strain. An animal study indicated that the ChiVax-WN01 chimera presented moderate safety and immunogenicity for 4-week female BALB/c mice.
Highlights
West Nile virus disease is a wide-spread arboviral zoonosis
A total of 2.5 μg of the chimeric West Nile virus infectious clones pChiVax-WN01 were directly transfected into the BSR/T7 cells grown in one well of 6-well plate with Lipofectamine 3000 (Thermo Scientific, Waltham, MA, USA) according to the transfection protocols
In order to acquire the chimeric infectious clone, prMΔE gene of Japanese encephalitis virus (JEV) SA14-14-2 w replaced with that of the West Nile virus (WNV) NY99 based on the JEV SA14-14-2 infectious clone pFLJE
Summary
West Nile virus disease is a wide-spread arboviral zoonosis. The clinical manifestation varies from nonspecific febrile illness and can progress to more severe hemorrhagic fever or encephalitis [1]. West Nile virus disease was sporadic in the areas of Africa, Europe, and west Asia, at first Later, it spread to the United States in 1999 [3]. The clinical manifestations of infection vary considerably from nonspecific febrile illness to more severe syndromes, including hemorrhagic fever, encephalitis, or microcephaly. Due to the better safety and protection, two licensed live-attenuated vaccines (YFV 17D and JEV SA14-14-2) have been used as vectors to construct recombinant flavivirus. Li XF (2013) developed a chimeric virus called ChinWNV, which replaced the prM∆E gene of JEV SA14-14-2 backbone with that of WNV strain Chin-01. Considering the lower neurovirulence of JEV SA14-14-2 and lower side effects, we employed JEV SA14-14-2 as a backbone to construct chimeric virus for further viral research. The variety of chimeric virus research may confer many more vaccine reservoirs
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