Abstract
ABSTRACTThirty semen ejaculates collected from five Large White Yorkshire boars were preserved in Beltsville thawing solution (BTS) and SAFEcell plus extenders at 16–18°C over a period of seven days. Preserved semen samples were evaluated for sperm motility, plasma membrane integrity and concentration of lipid peroxidation compound malondialdehyde (MDA) every day during storage. Semen samples preserved in the SAFEcell plus extender had significantly more per cent of sperm cells with total motility, progressive forward motility and plasma membrane integrity, significantly low level of MDA concentration than BTS extender from day 1 to day 7 of storage. It can be concluded that the SAFEcell plus extender is better than BTS to preserve boar semen in the liquid state at 16–18°C.
Highlights
Artificial insemination (AI) is a well-adapted biotechnological tool in bovine species
Preserved semen samples were evaluated for sperm motility, plasma membrane integrity and concentration of lipid peroxidation compound malondialdehyde (MDA) every day during storage
Semen samples preserved in the SAFEcell plus extender had significantly more per cent of sperm cells with total motility, progressive forward motility and plasma membrane integrity, significantly low level of MDA concentration than Beltsville thawing solution (BTS) extender from day 1 to day 7 of storage
Summary
Artificial insemination (AI) is a well-adapted biotechnological tool in bovine species. Metabolic activities of sperm cells continued during storage at 18°C results in the exhaust of available nutrients and accumulation of metabolic end products including reactive oxygen species (ROS) in the semen. Seminal plasma is endowed with antioxidant capacity (Jimenez et al 1990) which has the property of scavenging ROS and protect spermatozoa against oxidative stress. It is essential to find out the suitable extenders for preservation of boar semen in the liquid condition which may maintain fertilizing potential of sperm cells. The study was conducted to evaluate the efficiency of two commercially available extenders to preserve boar semen at 16–18°C in the liquid state in terms of sperm cell motility, plasma membrane integrity and lipid peroxidation (LPO) status
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