Abstract
The characteristics of ATP-activated currents ( I ATP) in rat nodose ganglion (NG) neurons have not been fully clarified. Especially, the correlation between I ATP phenotype and P2X receptor subunit genotype in rat NG neuron is not clear. By whole-cell patch-clamp and single cell immunocytochemical techniques, we explored the characteristics of the I ATP phenotype and its correlation with P2X receptor subunits in acutely isolated NG neuron of rats. The results indicated that I ATP of NG neurons can be classified into four types: F type (fast type, 8.1%), I type (intermediate type, 14.8%), S type (slowing type, 37.0%) and vS type (very slowing type, 21.5%). The single immunocytochemical studies have demonstrated that F type cells express P2X1 and P2X3 subunits, I type cells P2X1, P2X3 and P2X4 subunits, S type cells P2X1, P2X2 and P2X3 subunits and vS type cells P2X2, P2X3 and P2X4 subunits. The results reveal that there are four types of I ATP in NG neurons, differential expression of distinct P2X subunits may underlie the I ATP phenotype.
Published Version
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