Characteristics of acute rejection model of DA to LEW orthotopic liver transplantation in inbred rats

Abstract

Objective To study the characteristics of liver transplantation models in inbred rats DA to LEW and judge the grades of acute rejection. Methods Surgical skills were trained by using closed colony SD and Wistar rats, followed by DA to LEW orthotopic liver transplantation with Kama-da's two-cuff technique. The grades of acute rejection were judged by their clinical manifestations and Banff schema. Results Fifteen models of DA to LEW liver transplantation were established. The suc-cessful rate was 86.7 %. The causes of death were hemorrhage and thrombosis of suprahepatic and in-frahepatic vena cava and obstruction of biliary tract. Grade I rejection occurred on the 3rd d and reached the peak after the 7th d after liver transplantation. Remaining models died of grade Ⅲ rejec-tion besides postoperative complication within 12 d after the transplantation. Conclusion The acute rejection of DA to LEW orthotopic liver transplantation models is stable and intensive. It can be effec-tively used to study allograft rejection and tolerance. But it is difficult to establish this kind of ortho-topic liver transplantation models in inbred rats because of their own tissue characteristics. Key words: Liver transplantation; Inbred rat; Model; Rejection