Induction of chemical hepatocarcinogenesis and establishment of orthotopic liver transplantation model with hepatocarcinoma in rats

Abstract

To explore the inducing method for hepatic carcinoma in rats and the operative technique in establishment of orthotopic liver transplantation (OLT) model in rats with induced hepatic tumor. Hepatic carcinoma was induced by diethylnitrosamine (DENA) in rats. Then OLT was performed, using a two-cuff vessel anastomosis method modified from that introduced by Kamada, with reconstruction of hepatic artery. At the observation time point (18 weeks) after the initiation of carcinogenesis, the one-month survival rate in OLT group was higher than that in non-OLT group, 56.3% and 21.4% respectively, P < 0.05. The operative successful rate in tumor rats group and control group was 87.5% and 90.0%, respectively. In OLT rats with hepatic carcinoma group, metastasis mainly occurred in lung and abdomen, while only 2 cases of intrahepatic recurrence were observed. There was significant difference between the tumor rats group and control group in cum survival rate. DENA can wholesale induce stable rat model for hepatic carcinoma. OLT can markedly elevate survival rate in rats with liver cancer. In rats with induced hepatic tumor, OLT model, suggested by the author, was established with hepatic arterial reconstruction and it offers a satisfied method with more similar pattern of physiological setting and carcinoma development situation.