CHARACTERISTIC OF NON-SULFUR PURPLE BACTERIA RHODOPSEUDOMONAS PALUSTRIS FROM LAKE JAVORIVSKE (UKRAINE)

Abstract

The аіm of work was isolation from water of lake Javorivske, identification and investigation of purple non-sulfur bacteria with ability to use sulfide and grow on the organic compounds. Methods. Bacteria were cultivated under anaerobic and microaerophilic conditions, on the light or in the dark in the Van Niel or PNSB media with addition of different electron donors, carbon and nitrogen sources. Biomass was determined using turbidity diluted cell suspension photometration on CPK-3. The concentration of S2- was determined colorimetrically for methylen blue formation. The elemental sulfur extracted from medium used filtration through nitrocellulose filters. The sulfur concentration was measured by the method of Van Gemerden. For analyses of pigment content it has been used spectrophotometer Specord M-40. Bacterial cell structure was investigated with an electron transmission microscope PEM-100. The fragments primary structure was determined on GATC Biotech AG (Konstanz, Germany). The search for homologues to our bacteria used the BLASTN-program. Results. Te bacteria cells are rod-shaped and motile. Photosyntetical membranes are of lamellar type. The main photosyntetical pigments are bacteriachlorophyll a and carotenoids of spirilloxantin series. Bacteria grow chemotrophycally or phototrophycally, use sulfide as an electron donor and produce elemental sulfur, which extracted in cultural medium. Bacteria use different electron donors, carbone and nitrogen sources. They are facultative anaerobes. Bacteria were genetically similar to Rhodopseudomonas faecalis (99%), Rhodopseudomonas palustris (98%) and Rhodopseudomonas rhenobacensis (98%). Conclusions. Purple bacteria isolated from water of lake Javorivske were identified as Rhodopseudomonas palustris. They use sulfide and grow well due to utilization of organic compounds.