Abstract
Two isoforms of peroxisome proliferator-activated receptor gamma (PPARγ) cDNAs, γ1 and γ2, have been isolated and characterised in swine. The relative expression of the two transcripts was studied by northern blot analysis using total RNA isolated from several porcine tissues taken at three different ages (day 1, after 5 weeks and at 100 kg weight). Hybridisation were carried out with two different probes, one binding to both PPARγ transcripts and the other being PPARγ2 specific. Strongest hybridisation signals with the PPARγ probe binding both variants were detected in adipose tissues and spleen at all three ages, whereas only faint or no signals were detected in other tissues. The tissue distribution pattern of PPARγ1 and γ2 suggests a modulation of tissue distribution for the two transcripts and obvious age and breed differences in gene expression in swine.
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