Abstract
Bacteriophage has the characteristic property of clearing young broth cultures, either temporarily or permanently, and of forming clear areas in young cultures on solid media. These clear zones have been styled “taches vierges,” plaques or colonies; they are considered by D’Herelle to represent colonies of the virus. Single, discrete, bacterial colonies present a nibbled appearance. There is no such effect on killed organisms, which can adsorb the phage. The action of a phage might not be confined to one particular organism; when several organisms are acted upon by a phage, the action is unequal and tends to be more marked against certain organisms than others. Bacteriophage is widely distributed in nature. It has been isolated with great frequency from the feces of man and animals, sewage, water, and soil. It has also been obtained from samples of pus, and some workers claim that it can be generated spontaneously by serial cultivation of an organism in its own broth filtrate.
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