Abstract
Digitization is the process that generates a digital image by measuring and quantizing an optical image. Display is the process of generating a visible image by interpolating a digital image. A number of factors affect the quality of the digital and displayed images produced. The different components in the imaging chain should be well matched to each other and to the projects that the system will be expected to support. Factors that can degrade image quality in the digitizing process are loss of detail, noise, aliasing, shading, photometric nonlinearity, and geometric distortion. Factors that affect displayed image quality include displayed image size (native resolution), pixel size and shape, photometric linearity, and noise. The resolution of a microscope image digitizer is limited by the pixel size and spacing and by the wave nature of light (the diffraction limit). Noise tends to reduce the effective photometric resolution of both digitizers and displays. Geometric distortion affects the size, shape, and position of objects in an image.
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