Abstract
Although metalloproteins or metal-binding proteins have been ascertained to be associated with human health and diseases, the roles of metalloproteins in disease processes are not fully understood and require further comprehensive exploration. Immobilized metal affinity chromatography (IMAC) was initially developed for the purification of polyhistidine-tagged proteins, and subsequently for the analysis of phosphorylated proteins. Upon incorporation of different metal ions [e.g., Co(II), Cu(II)] onto solid resin, IMAC was further extended to track or enrich metal-binding proteins to elucidate their biological processes in various prokaryotic and eukaryotic cells. This chapter summarizes the progress of IMAC in metalloproteomics and phosphoproteomics, providing deeper insights into the mode of action of essential metal ions and metal-based drugs as well as the role of phosphorylated proteins in multiple cellular processes.
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