Abstract
Reversed-phase chromatography (RP-HPLC) is the most common HPLC separation technique and it is used for the separation of many types of compounds. The compounds amenable for RP-HPLC separation should have some (accessible) hydrophobic moieties in their molecule but the presence of polar groups does not preclude the use of RP-HPLC. Compounds with very different polarities can be analyzed by RP-HPLC. Based on octanol/water partition coefficient for polarity characterization, compounds with log Kow in the (approximate) range between −1.75 and +5.55 can be analyzed using RP-HPLC which includes many types of molecules.
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