Abstract

In this experiment, PCR primers aimed at amplifying short tandem repeat (STR) regions of DNA as selected from the Combined DNA Index System (CODIS) will be designed and tested in silico using freeware software including IDT’s Biotools and BLAST. After a single set of PCR primers are designed, primer multiplexes will be created to simultaneously amplify multiple DNA loci. This lab enables students to more fully understand how the primers and multiplex DNA typing kits routinely employed by the crime labs function and the engineering inherent in creating multiplexes.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call