Chapter 8 Instrumentation for liquid chromatography

Abstract

Publisher Summary This chapter discusses the instrumentation for liquid chromatography. The rapid development of the chemistry of natural products after the Second World War is directly connected with the utilization of chromatographic separation methods. The techniques of liquid chromatography play an important role in this development and form the basis of high-efficiency liquid chromatography. The extensive use of liquid column chromatography in the past has been evidently the result not only of the extension of the applicability of separation, analytical, and preparative methods but also of the simplicity and cheapness of chromatographic equipment. Currently, the classical procedures of column chromatography are still used predominantly and cannot be surpassed from the methodical point of view. More expensive fraction collectors sometimes have selectable regulation principles. Weight-regulated collectors have numerous disadvantages: the apparatus is sensitive to heating, the adjustment of balance is exacting, they often require vessels with identical weights, and they cannot be used if the density of the effluent changes.