Abstract
In sorghum, improvement in productivity and resistance to biotic and abiotic stresses were done using traditional breeding approaches. Novel genes, beyond primary and secondary gene pools, have potential to enhance overall sorghum productivity through genetic modifications in sorghum. However, sorghum crop improvement through genetic engineering is a challenging task. Sorghum is extremely recalcitrant to produce transgenic plants in vitro. Standardization of every technique, starting from selection of explants to regeneration of transgenic plants, is important in developing a successful and commercial event. Till date, many sorghum transgenics were reported using particle bombardment and Agrobacterium-mediated methods by making use of shoot apical meristems and immature embryos as the explant material. Successful application of these genetic transformation studies would open up the avenue for sorghum genotypes with superior traits. Basic concepts and essential factors required for transferring needful traits to sorghum using potential genetic manipulation approaches are discussed in this chapter.
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